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Tuesday, 16 January 2018

Agen Biologi 5




Khasiat pokok semambu

Assesssment the molluscicidal properties of azadirachtin against golden apple snail, Pomacea canaliculata



Abstract


Concern with the negative impact of synthetic pesticide on environment and human health have led this study in order to evaluate the molluscicidal efficacies of azadirachtin in neem seed crude extract on golden apple snail.

Azadirachtin was extracted by maceration technique using four different solvents and the quantity of azadirachtin in extracts was compared to select the best solvent.

Then, bioassays were performed on adult of golden apple snail to compare the molluscicidal activity of azadirachtin.

A comparison of the extractive yields of different solvents indicated that the polarity of the solvents tested not significantly influence in enhanced the azadirachtin yields.

The result on mortality rate of golden apple snail subjected to various concentration and solvent extracts indicated that neem seed crude extracts significantly killed the golden apple snail.

The LC50 values of the methanol extract (21.008mg/ml) were the lowest, indicating the highest potency, followed in order by ethanol extract (43.726mg/ml), acetone extract (48.110mg/ml) and water extract (53.654mg/ml).

 The mortality rate was correlated positively with the extract concentrations as the mortality of snail increased with the increase of extract concentration.

Therefore, this study indicated that neem seed crude extracts possessed molluscicidal effect for controlling the golden apple snail

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