Melioidosis

Melioidosis

melioidosis..transmission

People can get Melioidosis through direct contact with contaminated soil and surface waters.

Humans and animals are believed to acquire the infection by inhalation of contaminated dust or water droplets, ingestion of contaminated water, and contact with contaminated soil, especially through skin abrasions.

It is very rare for people to get the disease from another person. While a few cases have been documented, contaminated soil and surface water remain the primary way in which people become infected.

Besides humans, many animal species are susceptible to melioidosis, including:

·         Sheep

·         Goats

·         Swine

·         Horses

·         Cats

·         Dogs

·         Cattle

melioidosis.signs-symptoms

There are several types of melioidosis infection, each with their own set of symptoms.

However, it is important to note that melioidosis has a wide range of signs and symptoms that can be mistaken for other diseases such as tuberculosis or more common forms of pneumonia.

Localized Infection:

·         Localized pain or swelling

·         Fever

·         Ulceration

·         Abscess

Pulmonary Infection:

·         Cough

·         Chest pain

·         High fever

·         Headache

·         Anorexia

Bloodstream Infection:

·         Fever

·         Headache

·         Respiratory distress

·         Abdominal discomfort

·         Joint pain

·         Disorientation

Disseminated Infection:

·         Fever

·         Weight loss

·         Stomach or chest pain

·         Muscle or joint pain

·         Headache

·         Seizures

The time between an exposure to the bacteria that causes the disease and the emergence of symptoms is not clearly defined, but may range from one day to many years; generally symptoms appear two to four weeks after exposure.

Although healthy people may get melioidosis, the major risk factors are:

·         Diabetes

·         Liver disease

·         Renal disease

·         Thalassemia

·         Cancer or another immune-suppressing condition not related to HIV

·         Chronic Lung disease (such as cystic fibrosis, chronic obstructive pulmonary disease (COPD), and bronchiectasis)

melioidosis.risk

While melioidosis infection has taken place all over the world, Southeast Asia and northern Australia are the areas in which it is primarily found.

In the United States, confirmed cases reported in previous years have ranged from zero to five and have occurred among travelers and immigrants coming from places where the disease is widespread.

Moreover, it has been found among troops of all nationalities that have served in areas with widespread disease.

The greatest numbers of melioidosis cases are reported in:

·         Thailand

·         Malaysia

·         Singapore

·         Northern Australia

Though rarely reported, cases are thought to frequently occur in:

·         Papua New Guinea

·         Most of the Indian subcontinent

·         Southern China

·         Hong Kong

·         Taiwan

·         Vietnam

·         Indonesia

·         Cambodia

·         Laos

·         Myanmar (Burma)

Outside of Southeast Asia and Australia, cases have been reported in:

·         The South Pacific (New Caledonia)

·         Sri Lanka

·         Mexico

·         El Salvador

·         Panama

·         Ecuador

·         Peru

·         Guyana

·         Puerto Rico

·         Martinique

·         Guadeloupe

·         Brazil

·         Parts of Africa and the Middle East

melioidosis.Diagnosis

Diagnosis

Melioidosis is diagnosed by isolating Burkholderia pseudomallei from blood, urine, sputum, skin lesions, or abscesses; or by detecting an antibody response to the bacteria.

Infection Classifications

Melioidosis can be categorized as an acute or localized infection, acute pulmonary infection, acute bloodstream infection, or disseminated infection. Sub-clinical infections are also possible. The incubation period (time between exposure and appearance of clinical symptoms) is not clearly defined, but may range from one day to many years; generally symptoms appear two to four weeks after exposure. Although healthy people may get melioidosis, the major risk factors are diabetes, liver disease, renal disease, thalassemia, cancer or another immune-suppressing condition not related to HIV.

Localized Infection

This form generally presents as an ulcer, nodule, or skin abscess and may result from inoculation through a break in the skin and may produce fever and general muscle aches. The infection may remain localized, or may progress rapidly through the bloodstream.

Pulmonary Infection

This is the most common form of presentation of the disease and can produce a clinical picture of mild bronchitis to severe pneumonia. The onset of pulmonary melioidosis typicall is marked by a high fever, headache, anorexia, and general muscle soreness. Chest pain is common, but a nonproductive or productive cough with normal sputum is the hallmark of this form of melioidosis. Cavitary lesions may be seen on chest X-ray, similar to those seen in pulmonary tuberculosis.

Bloodstream Infection

Patients with underlying risk factors such as diabetes and renal insufficiency are more likely to develop this form of the disease, which usually results in septic shock. The symptoms of bloodstream infection may include fever, headache, respiratory distress, abdominal discomfort, joint pain, muscle tenderness, and disorientation. This is typically an infection with rapid onset, and abscesses may be found throughout the body, most notably in the liver, spleen, or prostate.

Disseminated Infection

Disseminated melioidosis presents with abscess formation in various organs of the body, and may or may not be associated with sepsis. Organs involved typically include the liver, lung, spleen, and prostate; involvement of joints, bones, viscera, lymph nodes, skin, or brain may also occur. Disseminated infection may be seen in acute or chronic melioidosis. Signs and symptoms, in addition to fever, may include weight loss, stomach or chest pain, muscle or joint pain, and headache or seizure.

melioidosis.prevention

In areas where the disease is widespread (see map below), contact with contaminated soil or water can put people at risk for melioidosis.

However, in these areas, there are things that certain groups of people can do to help minimize the risk of exposure:

·         Persons with open skin wounds and those with diabetes or chronic renal disease are at increased risk for melioidosis and should avoid contact with soil and standing water.

·         Those who perform agricultural work should wear boots, which can prevent infection through the feet and lower legs.

·         Health care workers can use standard contact precautions (mask, gloves, and gown) to help prevent infection.

Penyakit dari Tanah

Penyakit dari Tanah

Melioidosis

Melioidosis is an infectious disease caused by a Gram-negative bacterium, Burkholderia pseudomallei, found in soil and water. It is of public health importance in endemic areas, particularly in Vietnam and northern Australia. It exists in acute and chronic forms. Signs and symptoms may include pain in chest, bones, or joints; cough; skin infections, lung nodules, and pneumonia.

B. pseudomallei was previously classed as part of the Pseudomonas genus; until 1992, it was known as Pseudomonas pseudomallei. It is phylogenetically related closely to Burkholderia mallei which causes glanders, an infection primarily of horses, donkeys, and mules. The name melioidosis is derived from the Greek melis (μηλις) meaning "a distemper of asses" with the suffixes -oid meaning "similar to" and -osis meaning "a condition", that is, a condition similar to glanders.^[1]

Acute melioidosis

 

In the subgroup of patients where an inoculating event was noted, the mean incubation period of acute melioidosis was 9 days (range 1–21 days).^[2] Patients with latent melioidosis may be symptom-free for decades; the longest period between presumed exposure and clinical presentation is 62 years.^[3] The potential for prolonged incubation was recognized in US servicemen involved in the Vietnam War, and was referred to as the "Vietnam time-bomb". A wide spectrum of severity exists; in chronic presentations, symptoms may last months, but fulminant infection, particularly associated with near-drowning, may present with severe symptoms over hours.

A patient with active melioidosis usually presents with fever. Pain or other symptoms may be suggestive of a clinical focus, which is found in around 75% of patients. Such symptoms include cough or pleuritic chest pain suggestive of pneumonia, bone or joint pain suggestive of osteomyelitis or septic arthritis, or cellulitis. Intra-abdominal infection (including liver and/or splenic abscesses, or prostatic abscesses) do not usually present with focal pain, and imaging of these organs using ultrasound or computed tomography should be performed routinely. In one series of 214 patients, 27.6% had abscesses in the liver or spleen (95% confidence interval, 22.0% to 33.9%). B. pseudomallei abscesses may have a characteristic "honeycomb" or "swiss cheese" architecture (hypoechoic, multiseptate, multiloculate) on CT.^[4][5]

Regional variations in disease presentation are seen: parotid abscesses characteristically occur in Thai children, but this presentation has only been described once in Australia.^[6] Conversely, prostatic abscesses are found in up to 20% of Australian males, but are rarely described elsewhere. An encephalomyelitis syndrome is recognised in northern Australia.

Patients with melioidosis usually have risk factors for disease, such as diabetes, thalassemia, hazardous alcohol use, or renal disease, and frequently give a history of occupational or recreational exposure to mud or pooled surface water.^[7] However, otherwise healthy patients, including children, may also get melioidosis.

In up to 25% of patients, no focus of infection is found and the diagnosis is usually made on blood cultures or throat swab. Melioidosis is said to be able to affect any organ in the body except the heart valves (endocarditis). Although meningitis has been described secondary to ruptured brain abscesses, primary meningitis has not been described. Less common manifestations include intravascular infection, lymph node abscesses (1.2–2.2%),^[8] pyopericardium and myocarditis, mediastinal infection, and thyroid and scrotal abscesses and ocular infection.

 

Chronic melioidosis

 

Chronic melioidosis is usually defined by a duration of symptoms greater than two months and occurs in about 10% of patients.^[9] The clinical presentation of chronic melioidosis is protean and includes such presentations as chronic skin infections, chronic lung nodule, and pneumonia. In particular, chronic melioidosis closely mimics tuberculosis, and has sometimes been called "Vietnamese tuberculosis".^[10][11][12]

A definitive diagnosis is made by culturing the organism from any clinical sample, because the organism is never part of the normal human flora.

A definite history of contact with soil may not be elicited, as melioidosis can be dormant for many years before manifesting.^[13] Attention should be paid to a history of travel to endemic areas in returned travellers. Some authors recommend considering possibility of melioidosis in every febrile patient with a history of traveling to and/or staying at endemic areas.

A complete screen (blood culture, sputum culture, urine culture, throat swab, and culture of any aspirated pus) should be performed on all patients with suspected melioidosis (culture on blood agar as well as Ashdown's medium). A definitive diagnosis is made by growing B. pseudomallei from any site. A throat swab is not sensitive, but is 100% specific if positive, and compares favourably with sputum culture.^[14] The sensitivity of urine culture is increased if a centrifuged specimen is cultured, and any bacterial growth should be reported (not just growth above 10⁴ organisms/ml which is the usual cutoff).^[15] Very occasionally, bone marrow culture may be positive in patients who have negative blood cultures for B. pseudomallei, but these are not usually recommended.^[16] A common error made by clinicians unfamiliar with melioidosis is to only send a specimen from the affected site (which is the usual procedure for most other infections) instead of sending a full screen.

Ashdown's medium, a selective medium containing gentamicin, may be required for cultures taken from nonsterile sites. Burkholderia cepacia medium may be a useful alternative selective medium in nonendemic areas, where Ashdown's is not available.^[17] A new medium derived from Ashdown, known as Francis medium, may help differentiate B. pseudomallei from B. cepacia and may help in the early diagnosis of melioidosis,^[18] but has not yet been extensively clinically validated.

Many commercial kits for identifying bacteria may misidentify B. pseudomallei (see Burkholderia pseudomallei for a more detailed discussion of this topic).

A serological test for melioidosis (indirect haemagglutination) is available, but not commercially in most countries. A high background titre may reduce the positive predictive value of serological tests in endemic countries. A specific direct immunofluorescent test and latex agglutination, based on monoclonal antibodies, are used widely in Thailand, but are not available elsewhere. Cross-reactivity with B. thailandensis is almost complete.^[19] A commercial ELISA kit for melioidosis appears to perform well.^[20] but no ELISA test has yet been clinically validated as a diagnostic tool.^[21]

It is not possible to make the diagnosis on imaging studies alone (X-rays and scans),^[22] but imaging is routinely performed to assess the full extent of disease.^[23] Imaging of the abdomen using CT scans or ultrasound is recommended routinely, as abscesses may not be clinically apparent and may coexist with disease elsewhere. Australian authorities suggest imaging of the prostate specifically due to the high incidence of prostatic abscesses in northern Australian patients. A chest X-ray is also considered routine, with other investigations as clinically indicated. The presence of honeycomb abscesses in the liver are considered characteristic, but are not diagnostic.^[22][23]

The differential diagnosis is extensive; melioidosis may mimic many other infections, including tuberculosis.^[10]

Current treatment[edit]

The treatment of melioidosis is divided into two stages, an intravenous high-intensity phase and an eradication phase to prevent recurrence.

Intravenous intensive phase

Intravenous ceftazidime is the current drug of choice for treatment of acute melioidosis.^[24][25] Meropenem,^[26] imipenem^[2] and the cefoperazone-sulbactam combination (Sulperazone)^[27] are also active.^[28] Intravenous amoxicillin-clavulanate (co-amoxiclav) may be used if none of the above four drugs is available, but it produces inferior outcomes.^[29] Intravenous antibiotics are given for a minimum of 10 to 14 days, and are not usually stopped until the patient's temperature has returned to normal for more than 48 hours. Even with appropriate antibiotic therapy, fevers often persist for weeks or months, and patients may continue to develop new lesions even while on appropriate treatment. The median fever clearance time in melioidosis is 10 days:^[29] and failure of the fever to clear is not a reason to alter treatment. It is not uncommon for patients to require parenteral treatment continuously for a month or more.

Intravenous meropenem is routinely used in Australia;^[9] outcomes appear to be good and meropenem is currently being tested with ceftazidime in a Thai clinical trial.^[30]

Theoretical reasons are given for believing mortality might be lower in patients treated with imipenem: first, less endotoxin is released by dying bacteria during imipenem treatment,^[31] and the minimum inhibitory concentration (MIC) for imipenem is lower than for ceftazidime. However, no clinically relevant difference was found in mortality between imipenem and ceftazidime treatments.^[2] The MIC of meropenem is higher for B. pseudomallei than for many other organisms, and patients being haemofiltered will need more frequent or higher doses.^[32]

Moxifloxacin, cefepime, tigecycline, and ertapenem do not appear to be effective in vitro.^[33][34] Piperacillin-sulbactam ,^[33] doripenem and biapenem^[34][35] appear to be effective in vitro, but no clinical experience exists on which to recommend their use.

Adjunctive treatment with granulocyte colony-stimulating factor^[36] or co-trimoxazole^[37][38] were not associated with decreased fatality rates in trials in Thailand.

Eradication phase

Following the treatment of the acute disease, eradication (or maintenance) treatment with co-trimoxazole and doxycycline is recommended to be used for 12 to 20 weeks to reduce the rate of recurrence.^[39] Chloramphenicol is no longer routinely recommended for this purpose. Co-amoxiclav is an alternative for those patients who are unable to take co-trimoxazole and doxycycline (e.g., pregnant women and children under the age of 12),^[40][41] but is not as effective. Single agent treatment with a fluoroquinolone (e.g., ciprofloxacin)^[42][43] or doxycycline^[44] for the oral maintenance phase is ineffective.^[45]

In Australia, co-trimoxazole is used on its own for eradication therapy,^[9] with relapse rates that are lower than those seen in Thailand; in vitro evidence also suggests co-trimoxazole and doxycycline are antagonistic, and co-trimoxazole on its own may be preferable.^[46] Results from a randomised controlled trial (MERTH) support the use of co-trimoxazole alone.^[47] Studies reinforce the need for adequate follow-up and good adherence to the eradication phase of therapy. Dosing for co-trimoxazole is based on weight: (<40 kg: 160/800 mg every 12 hours; 40–60kg: 240/1200 mg every 12 hours, >60 kg: 320/1600 mg every 12 hours).^[48]

Surgical treatment[edit]

Surgical drainage is usually indicated for prostatic abscesses and septic arthritis, may be indicated for parotid abscesses, and is not usually indicated for hepatosplenic abscesses. In bacteraemic melioidosis unresponsive to intravenous antibiotic therapy, splenectomy has been attempted, but only anecdotal evidence supports this practice.^[49]

Prognosis

Without access to appropriate antibiotics (principally ceftazidime or meropenem), the septicemic form of melioidosis exceeds 90% in mortality rate.^[51][52] With appropriate antibiotics, the mortality rate is about 10% for uncomplicated cases but up to 80% for cases with bacteraemia or severe sepsis. It seems certain that access to intensive care facilities is also important, and probably at least partially explains why total mortality is 20% in Northern Australia but 40% in Northeast Thailand. Response to appropriate antibiotic treatment is slow, with the average duration of fever following treatment being 5–9 days.^[53][54]

Recurrence occurs in 10 to 20% of patients, but with co-trimoxazole eradication therapy, this can be reduced to 4%.^[55] While molecular studies have established the majority of recurrences are due to the original infecting strain, a significant proportion of recurrences (perhaps up to a quarter) in endemic areas may be due to reinfection, particularly after two years.^[56] Risk factors include severity of disease (patients with positive blood cultures or multifocal disease have a higher risk of relapse), choice of antibiotic for eradication therapy (doxycycline monotherapy and fluoroquinolone therapy are not as effective), poor compliance with eradication therapy and duration of eradication therapy less than 8 weeks.^[45][57]

Prevention

Person-to-person transmission is exceedingly unusual;^[58][59][60] and patients with melioidosis should not be considered contagious. Lab workers should handle B. pseudomallei under BSL-3 isolation conditions,^[61] as laboratory-acquired melioidosis has been described.

In endemic areas, people (rice-paddy farmers in particular) are warned to avoid contact with soil, mud, and surface water where possible.^{[citation needed]} Case clusters have been described following flooding and cyclones and probably relate to exposure. Other case clusters have related to contamination of drinking water supplies. Populations at risk include patients with diabetes mellitus, chronic renal failure, chronic lung disease, or an immune deficiency of any kind. The effectiveness of measures to reduce exposure to the causative organism have not been established. A vaccine is not yet available.

Epidemiology

Melioidosis is endemic in parts of southeast Asia (including Thailand,^[67] Laos,^[68][69][70] Singapore,^[71] Brunei,^[72] Malaysia, Burma and Vietnam), China,^[73] Taiwan^[74][75] and northern Australia.^[53][76] Flooding can increase its extent, including flooding in central Australia.^[77] Multiple cases have also been described in Hong Kong and Brunei^[78] India,^{[79][80][81][82]} and sporadic cases in Central and South America,^[83][84][85] the Middle East, the Pacific and several African countries.^[86][87] Although only one case of melioidosis has ever been reported in Bangladesh,^[88] at least five cases have been imported to the UK from that country. Recent news reports indicate B. pseudomallei has been isolated from soil in Bangladesh,^[89] but this remains to be verified scientifically. This suggests melioidosis is endemic to Bangladesh and a problem of underdiagnosis or under-reporting exists there.^[90] most likely due to a lack of adequate laboratory facilities in affected rural areas. A high isolation frequency (percentage of positive soil samples) was found in east Saravan in rural Lao PDR distant from the Mekong River, thought by the investigators to be the highest geometric mean concentration in the world (about 464 (25-10,850 CFU/g soil).^[91]

A statistical model indicated that the incidence will be 165,000 cases per year in 2016 (95% confidence interval, 68,000 to 412,000), with 138,000 of those occurring in East and South Asia and the Pacific.^[92] About half of those cases will die. Northeast Thailand has the highest incidence of melioidosis recorded in the world (an average incidence of 12.7 cases per 100,000 people per year).^[93] In Northeast Thailand, 80% of children are positive for antibodies against B. pseudomallei by the age of 4;^[94] the figures are lower in other parts of the world.^{[95][96][97][98]}

Melioidosis is a recognised disease in animals, including cats,^[99] goats, sheep, and horses. Cattle, water buffalo, and crocodiles are considered to be relatively resistant to melioidosis despite their constant exposure to mud.^[100] An outbreak at the Paris Zoo in the 1970s ("L’affaire du jardin des plantes") was thought to have originated from an imported panda.^[101]

B. pseudomallei is normally found in soil and surface water; a history of contact with soil or surface water is, therefore, almost invariable in patients with melioidosis;^[53] that said, the majority of patients who do have contact with infected soil suffer no ill effects. Even within an area, the distribution of B. pseudomallei within the soil can be extremely patchy,^[102][103] and competition with other Burkholderia species has been suggested as a possible reason.^[104] Contaminated ground water was implicated in one outbreak in northern Australia.^[105] Also implicated are severe weather events such as flooding^[106] tsunamis^[107] and typhoons.^[108][109]

Based on whole genome sequencing, humans may play a role in moving B. pseudomallei from place to place.^[110]

The single most important risk factor for developing melioidosis is diabetes mellitus, followed by hazardous alcohol use, chronic kidney disease, and chronic lung disease. Other risk factors include thalassaemia, occupation (rice paddy farmers),^[111] and cystic fibrosis.^[58][84] The mode of infection is believed to be either through a break in the skin, or through the inhalation of aerosolized B. pseudomallei cells. Person-to-person spread has been described, but is extremely unusual.^[58][59][60] HIV infection does not predispose to melioidosis.^{[112][113][114]}

The disease is clearly associated with increased rainfall, with the number (and severity) of cases increasing following increased precipitation